Active Gαs GTP  [30 µL] Catalog Number:  CR-M26906 Gene Symbol:  Gnas Description:   Anti Active Gαs GTP Mouse Monoclonal Antibody Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαsrepresents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases. Immunogen:   Recombinant full length protein of active Gαs Applications:   IP, IHC Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;   1:100 dilution for IHC Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Anti active Gαs antibody recognizes active Gαs of vertebrates Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles Immunoprecipitation/Western blot:


Gαs activation assay. Purified Gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti active Gαs monoclonal antibody (Cat. No. CR-M26906). Immunoblot was with an anti Gαsmonoclonal antibody (Cat. No. CR-M26006).