Starvio siRNA转染试剂是我公司研发团队在美国西雅图实验室研发成功的一种新型的小核酸转染试剂。Starvio siRNA可用来转染siRNA、miRNA mimics、miRNA inhibitor 等200bp以内的小分子RNA，转染细胞包括绝大多数贴壁生长的细胞，如一般细胞株、肿瘤细胞株等。目前，普通转染试剂的主要成分为脂质体或聚乙烯亚胺(Polyethylenimine, PEI)，这两种成分都具有很大的细胞毒性，并且转染效果很不理想。Starvio siRNA采用新型可降解纳米材料，毒性很低并且拥有非常高的转染性能，基因敲降效率可高达90%以上，转染细胞死亡率只有5%左右。血清对Starvio的转染效果没有影响，不必使用特殊的低血清培养基，也不必在转染4-6小时后更换培养液。本产品适用于贴壁细胞株转染，原代细胞转染，请选择StarvioPMsiRNA原代细胞转染试剂。

产品特点

· 极低的细胞毒性：转染细胞死亡率在5%左右，大大降低了因细胞毒性对实验结果的影响；

Fig. 1. Starvio siRNA transfection reagent offers the highest transfection efficiency.  HeLa cells were transfected with Alexa 546-dye labeled siRNA at final concentration of 10nM using Starvio sRNA transfection reagent. After 24 hrs, the cells were stained with Hoechst 33342 (nuclear staining dye) and then were viewed by fluorescence microscopy. A The fluorescence images show the fluorescence of labeled siRNA.B.The fluorescence images show the fluorescence of labeled siRNA (in red) overlaid with fluorescence from Hoechst 33342 nuclear staining (in blue).

Fig..2. Starvio siRNA transfection reagent offers the highest level of gene knockdown on a variety of cell types. Different cell lines were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using Starvio  In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

Fig..3.Starvio siRNA transfection reagent offers high level of gene knockdown with low toxicity. A549 cell lines were transfected with Lamin A/C siRNA (final concentration: 10nM) using Starvio siRNA transfection reagent. Starvio concentrations added to the cells  exceeding transfection concentration of 20 times, the cells still do not produce significant toxicity, which makes these product suitable to optimization. Lamin A/C knockdown was measured by qRT-PCR.

Fig.4.Starvio siRNA transfection reagent offers consistent high level of gene knockdown despite differences in cell density.A549 cells at different cell density were transfected with Lamin siRNA (final concentration: 10nM) using Starvio.

适用细胞

293T（人肾上皮细胞），A549（人肺癌细胞），HEK 293（人胚肾细胞），HeLa（人宫颈癌细胞），MCF-7（人乳腺癌细胞），HepG2（人肝肿瘤细胞），HT-1080(人纤维肉瘤），HLF-a（人肺细胞），Huh-7（人肝癌细胞），KB（人口腔表皮样癌细胞），PC3（人前列腺癌细胞），BRL-3A（大鼠正常肝细胞），DU 145（人前列腺癌细胞），Hepa 1-6（小鼠肝癌细胞），OVCAR3（人卵巢癌细胞），NCaP-FGC（人前列腺癌细胞），C2C12（成肌细胞），MDCK（犬肾细胞），CHO-K1，islk.219细胞（人肺癌细胞），HT-29（人结肠癌细胞），NCI-H1688（人典型小细胞肺肿瘤/癌细胞），RKO结肠癌细胞，Vero（非洲绿猴肾细胞），COS-1（非洲绿猴肾成纤维细胞），COS7-L（非洲绿猴肾成纤维细胞），COS-7（非洲绿猴肾成纤维细胞），SK-BR3（人乳腺癌细胞），Hepa 1cLc7（鼠肝癌细胞），Daoy（人髓母细胞瘤细胞），DBTRG-05MG（人脑胶质母细胞瘤细胞），KLN 205（鼠肺磷癌细胞），Neuro-2a（小鼠神经瘤母细胞），DI-TNC1（大鼠脑间质细胞）等。

相关文献

1.  Basic Research in Cardiology(2021). HIMF deletion ameliorates acute myocardial ischemic injury by promoting macrophage transformation to reparative subtype. (IF:11.981) 

2.   NUCLEIC ACIDS RESEARCH(2021). XPD localizes in mitochondria and protects the mitochondrial genome from oxidative DNA damage. (IF:11.24 ) 

3.  MYO1F regulates antifungal immunity by regulating acetylation of microtubules. (IF:11.205) 

4.  Cellular & Molecular Immunology(2021). The miR-628-5p Facilitates Enterovirus 71 Infection by Suppressing TRAF3-Mediated p65 and IRF3 Signaling. (IF:8.484)

5.  Levobupivacaine Induces Ferroptosis by miR-489-3p/SLC7A11 Signaling in Gastric Cancer. (IF:5.81)

6.  Proteome Analysis in PAM Cells Reveals That African Swine Fever Virus Can Regulate the Level of Intracellular Polyamines to Facilitate Its Own Replication through ARG1. (IF:5.046) 

7.  AGING-US(2021). Extracellular vesicles from anoxia preconditioned mesenchymal stem cells alleviate myocardial ischemia/reperfusion injury. (IF:4.909 ) 

8.  International Journal of Biological Sciences(2021).DGCR5 Promotes Gallbladder Cancer by Sponging MiR-3619-5p via MEK/ERK1/2 and JNK/p38 MAPK Pathways. (IF:4.773) 

9.  International Journal of Biological Sciences(2021).Rac GTPase activatingprotein 1 promotes gallbladder cancer via binding DNA ligase 3 to reduce apoptosis. (IF:4.773) DOI: 10.7150/ijbs.58857

10.  International Journal of Biological Sciences(2021).ESRRA promotes gastric cancer development by regulating the CDC25C/CDK1/CyclinB1 pathway via DSN1. (IF:4.773)  DOI:10.7150/ijbs.57623

11.  International Journal of Biological Sciences(2021).KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. (IF:4.773 )

12.  International Journal of Biological Sciences(2021). DGCR5 is activated by PAX5 and promotes pancreatic cancer via targeting miR-3163/TOP2A and activating Wnt/β-catenin pathway.(IF:4.773 ) DOI: 10.7150/ijbs.55636

13.  Journal of Cellular and Molecular Medicine(2021). Down‐ egulation of RCC1 sensitizes immunotherapy by up‐ egulating PD‐L1 via p27kip1/CDK4 axis in non‐small cell lung cancer. (IF:4.438) DOI: 

14.  scientific reports(2021). Clinicalsignificance of Annexin A2 e­xpression in oral squamous cell carcinoma and its influence on cell proliferation, migration and invasion. (IF:4.149) 

15.  Journal of Cancer(2021). LncRNALOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1. (IF:3.565) 

16.  Environmental Geochemistry and Health(2021).Inorganic arsenic influences cell apoptosis by regulating the e­xpression of MEG3 gene. (IF:3.472 ) 

17.  Bta-miR-223 Targeting CBLB Contributes to Resistance to Staphylococcus aureus Mastitis Through the PI3K/AKT/NF-κB Pathway. (IF:3.412) 

18.  Molecular Human Reproduction(2021).Excess fibronectin 1 participates in pathogenesis of pre-eclampsia by promoting apoptosis and autophagy in vascular endothelial cells. (IF:3.469) 

19.  American Journal of Translational Research(2021). Irg1-itaconateaxis protectsagainst acute kidney injury via activation of Nrf2. (IF:3.375 )

20.  ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH(2021).Arsenic exposure increased e­xpression of HOTAIR and LincRNA-p21 in vivo and vitro. (IF:3.33) 

21.  Environmental Toxicology and Pharmacology(2021).Long non-coding RNA DICER1-AS1-low e­xpression in arsenic-treated A549 cells inhibits cell proliferation by regulating the cell cycle pathway. (IF:3.292 )

22.  ENVIRONMENTAL TOXICOLOGY(2021).Inorganic arsenic‐mediated upregulation of AS3MT promotes proliferation of nonsmall cell lung cancer cells by regulating cell cycle genes. (IF:3.118 ) 

23.  Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells. (IF:2.59) 

24.  FEBS Open Bio(2021).SREBP1/FASN/cholesterol axis facilitates radioresistance in colorectal cancer.  (IF:2.231 ) 

25.  Clinical And Translational Medicine(2020).Long noncoding RNA lncGALM increases risk of liver metastasis in gallbladder cancer through facilitating N‐cadherin and IL‐1β‐dependent liver arrest and tumor extravasation. (IF:7.919 ) 

26.  Clinical And Translational Medicine(2020).Mir‐27a‐3p attenuates bronchiolitis obliterans in vivo via the regulation of dendritic cells' maturation and the suppression of myofibroblasts' differentiation. (IF:7.919 )

27.  Journal of Experimental & Clinical Cancer Research(2020).Loss of miR-192-5p initiates a hyperglycolysis and stemness positive feedback in hepatocellular carcinoma. (IF:7.068) 

28.  American Journal of Cancer Research(2020).ERRα promotes pancreatic cancer progression by enhancing the transcription of PAI1 and activating the MEK/ERK pathway.  (IF:5.884 )

29.   Frontiers in Cell and Developmental Biology(2020).CRLF1 is a key regulator in the ligamentum flavum hypertrophy. (IF:5.8 ) 

30.   Cancer Science(2020). Anthrax toxin receptor 1/tumor endothelial marker 8 promotes gastric cancer progression through activation of the PI3K/AKT/mTOR signaling pathway. (IF:4.985) 

31.  Journal of Cellular and Molecular Medicine(2020). Down‐ egulation of RCC1 sensitizes immunotherapy by up‐ egulating PD‐L1 via p27kip1/CDK4 axis in non‐small cell lung cancer. (IF:4.438 ) 

32.  Frontiers in Pharmacology(2020). Long Noncoding RNA LINC-PINT Suppresses Cell Proliferation, Invasion, and EMT by Blocking Wnt/β-Catenin Signaling in Glioblastoma. (IF:4.225) 

33.  Fish Shellfish Immunology(2020).Chlorpyrifos induces apoptosis and autophagy in common carp lymphocytes by influencing the TCR γ-dependent PI3K/AKT/JNK pathway. (IF:4.149) 

34.  Journal of Biological Chemistry(2020).Estrogen-induced FOS-like 1 regulates matrix metalloproteinase e­xpression and the motility of human endometrial and decidual stromal cells. (IF:3.311 ) DOI: 10.1074/jbc.RA119.010701

35.  Journal of Fish Diseases(2020).Chlorpyrifos induces redox imbalance‐dependent inflammation in common carp lymphocyte through dysfunction of T‐cell receptor γ.  (IF:2.323) 

36.  Molecular Medicine Reports(2020). Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3_x005f_x001e_E1 cells via an autocrine mechanism. (IF:2.085) 

37.  Chinese Medical Journal(2020).Topoisomerase IIalpha promotes gallbladder cancer proliferation and metastasis through activating phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway.  (IF:1.585) DOI: 10.1097/CM9.0000000000001075

38.  Autophagy(2019).USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy. (IF:11.06 )  DOI: 10.1080/15548627.2019.1656957

39.  Oxidative Medicine and Cellular Longevity(2019).Aloin preconditioning attenuates hepatic ischemia/reperfusion injury via inhibiting TLR4/MyD88/NF-κB signal pathway in vivo and in vitro.  (IF:5.163 ) DOI: 10.1155/2019/3765898

40.  Oxidative Medicine and Cellular Longevity.(2019)Toxicity of DON on GPx1-Overexpressed or Knockdown Porcine Splenic Lymphocytes In Vitro and Protective Effects of Sodium Selenite.(IF:5.076)DOI: 10.1155/2019/5769752

41.  Aging(2019).Circular RNA CircCCNB1 sponges micro RNA-449a to inhibit cellular senescence by targeting CCNE2. (IF:4.909 ) DOI: 10.18632/aging.102449

42.  International Journal of Biological Sciences(2019).Glycolytic enzyme PKM2mediates autophagic activation to promote cell survival in NPM1-mutated leukemia. (IF:4.773) DOI: 10.7150/ijbs.30290

43.  Cancer Science(2019).NOP2/Sun RNA methyltransferase 2 promotes tumor progression via its interacting partner RPL6 in gallbladder carcinoma. (IF:4.751 ) DOI: 10.1111/cas.14190

44.  International Journal of Molecular Sciences(2019).Comparison of LncRNA e­xpression profiles during myogenic differentiation and adipogenic transdifferentiation of myoblasts. (IF:4.602) DOI: 10.3390/ijms20153725

45.  Biomed Pharmacother(2019).LncRNA MNX1-AS1 promotes progression of esophageal squamous cell carcinoma by regulating miR-34a/SIRT1 axis. (IF:3.743) DOI: 10.1016/j.biopha.2019.109029

46.  Mediators of Inflammation(2019).miR-23b negatively regulates sepsis-induced inflammatory responses by targeting ADAM10 in human THP-1 monocytes. (IF:3.545)  DOI: 10.1155/2019/5306541

47.  Frontiers in Physiology(2019).Survival motor neuron (SMN) protein insufficiency exacerbates renal ischemia/reperfusion injury. (IF:3.367 ) DOI: 10.3389/fphys.2019.00559

48.  International Immunopharmacology(2019).MiR-150 attenuates LPS-induced acute lung injury via targeting AKT3. (IF:3.361 ) DOI: 10.1016/j.intimp.2019.105794

49.  Eur Rev Med Pharmacol Sci(2019). Long noncoding RNA PCAT6 regulates cell growth and metastasis via Wnt/beta-catenin pathway and is a prognosis marker in cervical cancer. (IF:2.727) 

50.  BioMed Research International(2019).PC-3-Derived ExosomesInhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-���B Signaling Pathway.  (IF:2.366) 

51.  Cancer Management and Research(2019).Long noncoding RNA FEZF1-AS1 promotes the motility of esophageal squamous cell carcinoma through Wnt/β-catenin pathway. (IF:2.243) 

52.  Molecular Medicine Reports(2019).BAFF is involvedin the pathogenesis of IgA nephropathy by activating the TRAF6/NF_x005fκB signaling pathway in glomerular mesangial cells. (IF:2.085)  

53.  Molecular Biology Reports(2019). Aberrant e­xpressionof long noncoding RNAs in the serum and myocardium of spontaneous hypertensive rats. (IF:1.466 ) 

54.  Journal of Experimental & Clinical Cancer Research(2018).INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia. (IF:5.189 ) 

55.  Journal of Endocrinology(2018). SRT2104attenuates diabetes-induced aortic endothelial dysfunction via inhibition of P53. (IF:4.706 ) 

56. scientific reports(2018). Sodium selenite inhibitsdeoxynivalenol-induced injury in GPX1-knockdown porcine splenic lymphocytes in culture. (IF:4.149)DOI: 10.1038/s41598-018-36149-x

57.  Biochimica et Biophysica Acta (BBA) - Molecular Cell Research(2018).MDM2 controls NRF2 antioxidant activity in prevention of diabetic kidney disease. (IF:3.982)  

58.  Oncotarget(2018).FC-99 ameliorates sepsis-induced liver dysfunction by modulating monocyte/macrophage differentiation via Let-7a related monocytes apoptosis. (IF:3.765)    

59.  Oncotarget(2018).Metformin synergistically suppress tumor growth with doxorubicin and reverse drug resistance by inhibiting the e­xpression and function of P-glycoprotein in MCF7/ADR cells and xenograft models. (IF:3.765) 

60.  Biochemistry and Cell Biology(2018).MMS19 localizes to mitochondria and protects the mitochondrial genome from oxidative damage. (IF:2.25 ) 

61.  Biochimica et Biophysica Acta(2018).A novel brown adipocyte-enrichedlong non-coding RNA that is required for brown adipocyte differentiation and sufficient to drive thermogenic gene program in white adipocytes. (IF:2.014 ) 

62.  Cell Research(2017).5-methylcytosine promotes mRNA export — NSUN2 as the  methyltransferase and ALYREF as an m5C reader. (IF:13.9 ) 

63.  Free Radical Biology & Medicine(2017).Epigallocatechin gallateupregulates NRF2 to prevent diabetic nephropathy via disabling KEAP1. (IF:6.078 ) 

64.  Journal of Molecular Endocrinology(2017).SP600125 suppresses Keap1 e­xpression and results in NRF2-mediated prevention of diabetic nephropathy. (IF:3.577) 

65.  Oncotarget(2016).Knockdown of Golgi phosphoprotein 2 inhibits hepatocellular carcinoma cell proliferation and motility. (IF:3.765 ) 

66.  Oncotarget(2016).Migration-inducing gene7 promotes tumorigenesis and angiogenesis and independently predicts poor prognosis of epithelial ovarian cance. (IF:3.765) 

67.  Molecular Nutrition Food Research(2014).Low‐dose diet supplement of a natural flavonoid, luteolin, ameliorates diet‐induced obesity and insulin resistance in mice. (IF:5.426)  

68.  scientific reports(2013). APG: an active protein-gene network model to quantify regulatory signals in complex biological systems. (IF:4.149)